Fiche du document
15 septembre 2022
- doi: 10.1242/jcs.260288
- issn: 0021-9533
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1242/jcs.260288
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/36148799
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1477-9137
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_E5AA6797B9AB3
info:eu-repo/semantics/openAccess , CC BY 4.0 , https://creativecommons.org/licenses/by/4.0/
Sujets proches
TropomyosinCiter ce document
T. Hatano et al., « mNG-tagged fusion proteins and nanobodies to visualize tropomyosins in yeast and mammalian cells. », Serveur académique Lausannois, ID : 10.1242/jcs.260288
Métriques
Partage / Export
Résumé
Tropomyosins are structurally conserved α-helical coiled-coil proteins that bind along the length of filamentous actin (F-actin) in fungi and animals. Tropomyosins play essential roles in the stability of actin filaments and in regulating myosin II contractility. Despite the crucial role of tropomyosin in actin cytoskeletal regulation, in vivo investigations of tropomyosin are limited, mainly due to the suboptimal live-cell imaging tools currently available. Here, we report on an mNeonGreen (mNG)-tagged tropomyosin, with native promoter and linker length configuration, that clearly reports tropomyosin dynamics in Schizosaccharomyces pombe (Cdc8), Schizosaccharomyces japonicus (Cdc8) and Saccharomyces cerevisiae (Tpm1 and Tpm2). We also describe a fluorescent probe to visualize mammalian tropomyosin (TPM2 isoform). Finally, we generated a camelid nanobody against S. pombe Cdc8, which mimics the localization of mNG-Cdc8 in vivo. Using these tools, we report the presence of tropomyosin in previously unappreciated patch-like structures in fission and budding yeasts, show flow of tropomyosin (F-actin) cables to the cytokinetic actomyosin ring and identify rearrangements of the actin cytoskeleton during mating. These powerful tools and strategies will aid better analyses of tropomyosin and F-actin cables in vivo.