High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets.

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2011

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Serveur académique Lausannois

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This document is linked to :
info:eu-repo/semantics/altIdentifier/doi/10.2337/db11-0726

This document is linked to :
info:eu-repo/semantics/altIdentifier/pmid/21926272

This document is linked to :
info:eu-repo/semantics/altIdentifier/eissn/1939-327X

This document is linked to :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_011499BBD0672

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Serveur Académique Lausannois

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Université de Lausanne et CHUV

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info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer

Keywords 0

Algorithms; Animals; Chlorides/administration & dosage; Contrast Media/administration & dosage; Diabetes Mellitus, Type 1/chemically induced; Diabetes Mellitus, Type 1/metabolism; Glucagon/metabolism; Glucagon-Secreting Cells/metabolism; Glucagon-Secreting Cells/pathology; Glucose/administration & dosage; Glucose/metabolism; Image Enhancement/methods; Immunohistochemistry; Infusions, Intravenous; Injections, Intraperitoneal; Insulin/metabolism; Insulin-Secreting Cells/metabolism; Insulin-Secreting Cells/pathology; Islets of Langerhans/metabolism; Islets of Langerhans/pathology; Magnetic Resonance Imaging; Manganese Compounds/administration & dosage; Mice; Mice, Inbred C57BL; Organ Size; Streptozocin/toxicity

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Mouse House mice Mus musculus House mouse Isles Islets

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S. Lamprianou et al., « High-resolution magnetic resonance imaging quantitatively detects individual pancreatic islets. », Serveur académique Lausannois, ID : 10.2337/db11-0726

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Abstract 0

OBJECTIVE-We studied whether manganese-enhanced high-field magnetic resonance (MR) imaging (MEHFMRI) could quantitatively detect individual islets in situ and in vivo and evaluate changes in a model of experimental diabetes.RESEARCH DESIGN AND METHODS-Whole pancreata from untreated (n = 3), MnCl(2) and glucose-injected mice (n = 6), and mice injected with either streptozotocin (STZ; n = 4) or citrate buffer (n = 4) were imaged ex vivo for unambiguous evaluation of islets. Exteriorized pancreata of MnCl(2) and glucose-injected mice (n = 6) were imaged in vivo to directly visualize the gland and minimize movements. In all cases, MR images were acquired in a 14.1 Testa scanner and correlated with the corresponding (immuno)histological sections.RESULTS-In ex vivo experiments, MEHFMRI distinguished different pancreatic tissues and evaluated the relative abundance of islets in the pancreata of normoglycemic mice. MEHFMRI also detected a significant decrease in the numerical and volume density of islets in STZ-injected mice. However, in the latter measurements the loss of beta-cells was undervalued under the conditions tested. The experiments on the externalized pancreata confirmed that MEHFMRI could visualize native individual islets in living, anesthetized mice.CONCLUSIONS-Data show that MEHFMRI quantitatively visualizes individual islets in the intact mouse pancreas, both ex vivo and in vivo. Diabetes 60:2853-2860, 2011

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