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1 septembre 2010
- handle: 10670/1.7eqvye
info:eu-repo/semantics/openAccess
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identification NIRS PCR peanut true hybridSujets proches
Field guides Keys (Identification guides) Identification guides Forensic identification Calvaries Crucifixes RoodsCiter ce document
Chuan Tang Wang et al., « Novel protocol to identify true hybrids in normal oleate x high oleate crosses in peanut », Electronic Journal of Biotechnology, ID : 10670/1.7eqvye
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A novel hybrid identification protocol was developed for F0:1 peanut seeds resulting from crosses between normal oleate cultivars with wild type FAD2B gene and high oleate genotypes with an A insertion in FAD2B gene. Presence of a series of overlapped peaks in trace file of the PCR product amplified with bF19/R1 primers was an indication of hybridity. This protocol may facilitate high oleate breeding and genetic studies in peanut.