Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites

Metadatas

Date

June 1, 2006

type
Language
Identifier
Organization

SciELO

License

info:eu-repo/semantics/openAccess


Keywords

ISSR ornamentals varietal identification


Cite this document

Mariana C. Pérez de la Torre et al., « Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites », Electronic Journal of Biotechnology, ID : 10670/1.8xuecp


Metrics


Share / Export

Abstract 0

The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular identification profile for the six new varieties. This result, supported together with our previous reports, indicates that the anchored microsatellites are a very useful technique for the fingerprints generation in N. linariaefolia.

From the same authors

On the same subjects

Similar documents

Within the same disciplines