Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.)

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Date

1 janvier 2008

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info:eu-repo/semantics/openAccess


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Argemone mexicana β-glucuronidase benzylisoquinoline alkaloids genetic transformation neomycin phosphotransferase II


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Gregorio Godoy-Hernández et al., « Agrobacterium-mediated transient transformation of Mexican prickly poppy (Argemone mexicana L.) », Electronic Journal of Biotechnology, ID : 10670/1.x54frg


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Shoot apex, leaf primordia, leaf sections and roots from Mexican prickly poppy seedlings, were inoculated with Agrobacterium tumefaciens harboring the binary vector pCAMBIA2301, which contained the β-glucuronidase (uid A) gene. Histochemical β-glucuronidase (GUS) assay in infected explants showed transient gus gene expression between 3 and 12 days after inoculation. To our knowledge, this is the first report of A. mexicana susceptibility to A. tumefaciens-mediated genetic transformation

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