Cytotoxic effect of Montivipera bornmuelleri's venom on cancer cell lines: In vitro and in vivo studies
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24 janvier 2025
- 10938/25168
- 2-s2.0-85094904729
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PeerJ
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Anti-cancer activity B16 cells In vivo Mca cells Montivipera bornmuelleri 3 methylcholanthrene Montivipera bornmuelleri venom Unclassified drug Venom Animal cell Animal experiment Animal model Animal tissue Article B16 cell line Cell proliferation Cell viability Controlled study Cutaneous melanoma Cytotoxicity Fibrosarcoma Flow cytometry Ic50 Immunomodulation In vitro study In vivo study Lebanon Male Melanoma Mouse Nonhuman Tumor growth Tumor volumeSujets proches
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Haddoub et al., « Cytotoxic effect of Montivipera bornmuelleri's venom on cancer cell lines: In vitro and in vivo studies », American University of Beirut ScholarWorks
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Background: Montivipera bornmuelleri's venom has shown immunomodulation of cytokines release in mice and selective cytotoxicity on cancer cells in a dose-dependent manner, highlighting an anticancer potential. Here, we extend these findings by elucidating the sensitivity of murine B16 skin melanoma and 3-MCAinduced murine fibrosarcoma cell lines to M. bornmuelleri's venom and its effect on tumor growth in vivo. Methods: The toxicity of the venom on B16 and MCA cells was assessed using flow cytometry and xCELLigence assays. For in vivo testing, tumor growth was followed in mice after intratumoral venom injection. Results: The venom toxicity showed a dose-dependent cell death on both B16 and MCA cells. Interestingly, overexpression of ovalbumin increased the sensitivity of the cells to the venom. However, the venom was not able to eradicate induced-tumor growth when injected at 100 μg/kg. Our study demonstrates a cytotoxic effect of M. bornmuelleri's venom in vitro which, however, does not translate to an anticancer action in vivo. © 2020 PeerJ Inc.. All rights reserved.
