Biomimetic sulfated glycosaminoglycans maintain differentiation markers of breast epithelial cells and preferentially inhibit proliferation of cancer cells
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24 janvier 2025
- 10938/27919
- 33444795.0
- 2-s2.0-85099634637
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Acta Biomaterialia
Mots-clés
Biomimetics Breast cancer Glycosaminoglycans Sulfated alginates Therapeutics Antigens, differentiation Cell proliferation Epithelial cells Humans Lung neoplasms Cells Controlled drug delivery Cytology Diseases Insulin Targeted drug delivery Alginate sulfate Antineoplastic agent Beta catenin Epidermal growth factor Fibroblast growth factor 2 Glycosaminoglycan polysulfate Heparin Unclassified drug A73025 Differentiation antigen Glycosaminoglycan Alginate-sulfate Cancer cells Epidermal growth factors Mammary epithelial cells Mimetics Sulfated glycosaminoglycans Sulphated alginate Therapeutic Antiproliferative activity Antitumorigenic activity Article Basement membrane Binding affinity Biotinylation Breast epithelium cell Cell differentiation Coculture Confocal laser scanning microscopy Controlled study Enzyme linked immunosorbent assay Hmt-3522 s1 cell line Human Human cell Immunofluorescence microscopy Immunofluorescence test Priority journal Protein localization Quartz crystal microbalance Sulfation Transwell assay Epithelium cell Lung tumor Sulfur compoundsSujets proches
Lung Carcinoma Cancers Malignancy (Cancer) Malignant tumors BreastsCiter ce document
Habli et al., « Biomimetic sulfated glycosaminoglycans maintain differentiation markers of breast epithelial cells and preferentially inhibit proliferation of cancer cells », American University of Beirut ScholarWorks
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Résumé
Glycosaminoglycans (GAG) are key elements involved in various physiological and pathological processes including cancer. Several GAG-based drugs have been developed showing significant results and potential use as cancer therapeutics. We previously reported that alginate sulfate (AlgSulf), a GAG-mimetic, reduces the proliferation of lung adenocarcinoma cells. In this study, we evaluated the preferential effect of AlgSulf on tumorigenic and nontumorigenic mammary epithelial cells in 2D, 3D, and coculture conditions. AlgSulf were synthesized with different degrees of sulfation (DSs) varying from 0 to 2.7 and used at 100 µg/mL on HMT-3522 S1 (S1) nontumorigenic mammary epithelial cells and their tumorigenic counterparts HMT-3522 T4–2 (T4–2) cells. The anti-tumor properties of AlgSulf were assessed using trypan blue and bromodeoxyuridine proliferation (BrdU) assays, immunofluorescence staining and transwell invasion assay. Binding of insulin and epidermal growth factor (EGF) to sulfated substrates was measured using QCM-D and ELISA. In 2D, the cell growth rate of cells treated with AlgSulf was consistently lower compared to untreated controls (p
