DNA degradation in avian faecal samples and feasibility of non-invasive genetic studies applied to threatened capercaillie populations
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2006
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info:eu-repo/semantics/altIdentifier/doi/10.1007/s10592-005-9023-7
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_1ABC56DED65D7
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S. Regnaut et al., « DNA degradation in avian faecal samples and feasibility of non-invasive genetic studies applied to threatened capercaillie populations », Serveur académique Lausannois, ID : 10.1007/s10592-005-9023-7
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We evaluated the feasibility of using faeces as a non-invasively collected DNA source for the genetic study of an endangered bird population (capercaillie; Tetrao urogallus). We used a multitube approach, and for our panel of 11 microsatellites genotyping reliability was estimated at 98% with five repetitions. Experiments showed that free DNases in faecal material were the major cause of DNA degradation. Our results demonstrate that using avian faeces as a source of DNA, reliable microsatellite genotyping can be obtained with a reasonable number of PCR replicates.