Synergistic effects of antimicrobial peptide dendrimer-chitosan polymer conjugates against Pseudomonas aeruginosa.

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Auteurs
Date

15 mars 2022

Type de document
Périmètre
Langue
Identifiants
Source

Serveur académique Lausannois

Relations

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.carbpol.2021.119025

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/35027127

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1879-1344

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_40DB7D6879C51

Collection

Serveur Académique Lausannois

Organisation

Université de Lausanne et CHUV

Licences

info:eu-repo/semantics/openAccess , CC BY-NC-ND 4.0 , https://creativecommons.org/licenses/by-nc-nd/4.0/

Mots-clés 0

Animals; Anti-Bacterial Agents/chemistry; Anti-Bacterial Agents/pharmacology; Anti-Bacterial Agents/toxicity; Antimicrobial Peptides/chemistry; Antimicrobial Peptides/pharmacology; Antimicrobial Peptides/toxicity; Cell Membrane/drug effects; Cells, Cultured; Chitosan; Dendrimers; Drug Synergism; Hemolysis; Humans; Microbial Sensitivity Tests; Polymers; Pseudomonas aeruginosa/drug effects; Antimicrobial formulations; Antimicrobial peptide dendrimers (AMPDs); Multidrug-resistant bacteria; Synergistic antimicrobial activities; Wound infections

Sujets proches En

Dendritic compounds

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V. Patrulea et al., « Synergistic effects of antimicrobial peptide dendrimer-chitosan polymer conjugates against Pseudomonas aeruginosa. », Serveur académique Lausannois, ID : 10.1016/j.carbpol.2021.119025

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We report herein a new chemical platform for coupling chitosan derivatives to antimicrobial peptide dendrimers (AMPDs) with different degrees of ramification and molecular weights via thiol-maleimide reactions. Previous studies showed that simple incorporation of AMPDs to polymeric hydrogels resulted in a loss of antibacterial activity and augmented cytotoxicity to mammalian cells. We have shown that coupling AMPDs to chitosan derivatives enabled the two compounds to act synergistically. We showed that the antimicrobial activity was preserved when incorporating AMPD conjugates into various biopolymer formulations, including nanoparticles, gels, and foams. Investigating their mechanism of action using electron and time-lapse microscopy, we showed that the AMPD-chitosan conjugates were internalized after damaging outer and inner Gram-negative bacterial membranes. We also showed the absence of AMPD conjugates toxicity to mammalian cells. This chemical technological platform could be used for the development of new membrane disruptive therapeutics to eradicate pathogens present in acute and chronic wounds.

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