Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches.
Fiche du document
18 août 2023
- doi: 10.1016/j.isci.2023.107261
- issn: 2589-0042
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.isci.2023.107261
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/37520703
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/2589-0042
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_50963F1342CA9
info:eu-repo/semantics/openAccess , CC BY-NC-ND 4.0 , https://creativecommons.org/licenses/by-nc-nd/4.0/
Sujets proches
Organisation Methods of analysis Analysis and chemistry Analytical methods Chemical analysis Analysis methods Analysis and examination Diseases--ImagingCiter ce document
B.L. Oyler et al., « Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary imaging approaches. », Serveur académique Lausannois, ID : 10.1016/j.isci.2023.107261
Métriques
Partage / Export
Résumé
Secondary human lymphoid tissue immune reactions take place in a highly coordinated environment with compartmentalization representing a fundamental feature of this organization. In situ profiling methodologies are indispensable for the understanding of this compartmentalization. Here, we propose a complementary experimental approach aiming to reveal different aspects of this process. The analysis of human tonsils, using a combination of single cell phenotypic analysis based on flow cytometry and multiplex imaging and mass spectrometry-based methodologies, revealed a compartmentalized organization at the cellular and molecular levels. More specifically, the skewed distribution of highly specialized immune cell subsets and relevant soluble mediators was accompanied by a compartmentalized localization of several lipids across different anatomical areas of the tonsillar tissue. The performance of such combinatorial experimental approaches could lead to the identification of novel in situ interactions and molecular targets for the in vivo manipulation of lymphoid organ, particularly the germinal center, immune reactions.