Sensitive photonic system to measure oxidative potential of airborne nanoparticles and ROS levels in exhaled air
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2015
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.proeng.2015.08.659
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info:eu-repo/semantics/altIdentifier/isbn/1877-7058
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_A4206687F4973
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Speciation (Biology) Oxygen radicals Reactive oxygen Singlet oxygen Superperoxide anionCiter ce document
Alexis Laulagnet et al., « Sensitive photonic system to measure oxidative potential of airborne nanoparticles and ROS levels in exhaled air », Serveur académique Lausannois, ID : 10.1016/j.proeng.2015.08.659
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A photonic system has been developed that enables sensitive quantitative determination of reactive oxygen species (ROS) - mainly hydrogen peroxide (H2O2) - in aerosol samples such as airborne nanoparticles and exhaled air from patients. The detection principle relies on the amplification of the absorbance under multiple scattering conditions due to optical path lengthening [1] and [2]. In this study, the presence of cellulose membrane that acts as random medium into the glass optical cell considerably improved the sensitivity of the detection based on colorimetric FOX assay (FeII/orange xylenol). Despite the loss of assay volume (cellulose occupies 75% of cell volume) the limit of detection is enhanced by one order of magnitude reaching the value of 9 nM (H2O2 equivalents). Spectral analysis is performed automatically with a periodicity of 5 to 15 s, giving rise to real-time ROS measurements. Moreover, the elution of air sample into the collection chamber via a micro-diffuser (impinger) enables quantitative determination of ROS contained in or generated from airborne samples. As proof-of-concept the photonic ROS detection system was used in the determination of both ROS generated from traffic pollution and ROS contained in the exhaled breath as lung inflammation biomarkers.