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- doi: 10.1016/j.tiv.2021.105129
- issn: 0887-2333
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tiv.2021.105129
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_65AA780564EB9
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E. Reale et al., « Influence of experimental parameters on in vitro human skin permeation of Bisphenol A. », Serveur académique Lausannois, ID : 10.1016/j.tiv.2021.105129
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Bisphenol A (BPA) in vitro skin permeation studies have shown inconsistent results, which could be due to experimental conditions. We studied the impact of in vitro parameters on BPA skin permeation using flow-through diffusion cells with ex-vivo human skin (12 donors, 3-12 replicates). We varied skin status (viable or frozen skin) and thickness (200, 400, 800 μm), BPA concentrations (18, 250 mg/l) and vehicle volumes (10, 100 and 1000 μl/cm 2 ). These conditions led to a wide range of BPA absorption (2%-24% after 24 h exposure), peak permeation rates (J = 0.02-1.31 μg/cm 2 /h), and permeability coefficients (K p = 1.6-5.2 × 10 -3 cm/h). This is the first time steady state conditions were reached for BPA aqueous solutions in vitro (1000 μl/cm 2 applied at concentration 250 mg/l). A reduction of the skin thickness from 800 and 400 μm to 200 μm led to a 3-fold increase of J (P < 0.05). A reduction of the vehicle volume from 1000 to 100 led to a 2-fold decrease in J (P > 0.05). Previously frozen skin led to a 3-fold increase in J compared to viable skin (P < 0.001). We found that results from published studies were consistent when adjusting J according to experimental parameters. We propose appropriate J values for different exposure scenarios to calculate BPA internal exposures for use in risk assessment.