Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.

L. Galluzzi; S.A. Aaronson; J. Abrams; E.S. Alnemri; D.W. Andrews; E.H. Baehrecke; N.G. Bazan; M.V. Blagosklonny; K. Blomgren; C. Borner; D.E. Bredesen; C. Brenner; M. Castedo; J.A. Cidlowski; A. Ciechanover; G.M. Cohen; V. De Laurenzi; R. De Maria; M. Deshmukh; B.D. Dynlacht; W.S. El-Deiry; R.A. Flavell; S. Fulda; C. Garrido; P. Golstein; M.L. Gougeon; D.R. Green; H. Gronemeyer; G. Hajnóczky; J.M. Hardwick; M.O. Hengartner; H. Ichijo; M. Jäättelä; O. Kepp; A. Kimchi; D.J. Klionsky; R.A. Knight; S. Kornbluth; S. Kumar; B. Levine; S.A. Lipton; E. Lugli; F. Madeo; W. Malomi; J.C. Marine; S.J. Martin; J.P. Medema; P. Mehlen; G. Melino; U.M. Moll; E. Morselli; S. Nagata; D.W. Nicholson; P. Nicotera; G. Nuñez; M. Oren; J. Penninger; S. Pervaiz; M.E. Peter; M. Piacentini; J.H. Prehn; H. Puthalakath; G.A. Rabinovich; R. Rizzuto; C.M. Rodrigues; D.C. Rubinsztein; T. Rudel; L. Scorrano; H.U. Simon; H. Steller; J. Tschopp; Y. Tsujimoto; P. Vandenabeele; I. Vitale; K.H. Vousden; R.J. Youle; J. Yuan; B. Zhivotovsky; G. Kroemer

Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.

Fiche du document

Auteurs

Date

2009

Types de document

Périmètre

Publications

Langue

Anglais

Identifiant

doi: 10.1038/cdd.2009.44

Source

Serveur académique Lausannois

Relations

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1038/cdd.2009.44

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/19373242

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pissn/1476-5403[electronic]

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_FFA980716F476

Collection

Serveur Académique Lausannois

Organisation

Université de Lausanne et CHUV

Licences

info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer

Mots-clés 0

Apoptosis; Cell Death; Eukaryotic Cells/cytology; Flow Cytometry; Guidelines as Topic; Humans; Immunoblotting; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Spectrometry, Fluorescence

Sujets proches En

Cell degeneration

Citer ce document

L. Galluzzi et al., « Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. », Serveur académique Lausannois, ID : 10.1038/cdd.2009.44

Partage / Export

Résumé 0

Cell death is essential for a plethora of physiological processes, and its deregulation characterizes numerous human diseases. Thus, the in-depth investigation of cell death and its mechanisms constitutes a formidable challenge for fundamental and applied biomedical research, and has tremendous implications for the development of novel therapeutic strategies. It is, therefore, of utmost importance to standardize the experimental procedures that identify dying and dead cells in cell cultures and/or in tissues, from model organisms and/or humans, in healthy and/or pathological scenarios. Thus far, dozens of methods have been proposed to quantify cell death-related parameters. However, no guidelines exist regarding their use and interpretation, and nobody has thoroughly annotated the experimental settings for which each of these techniques is most appropriate. Here, we provide a nonexhaustive comparison of methods to detect cell death with apoptotic or nonapoptotic morphologies, their advantages and pitfalls. These guidelines are intended for investigators who study cell death, as well as for reviewers who need to constructively critique scientific reports that deal with cellular demise. Given the difficulties in determining the exact number of cells that have passed the point-of-no-return of the signaling cascades leading to cell death, we emphasize the importance of performing multiple, methodologically unrelated assays to quantify dying and dead cells.

{"uri":"10.1038\/cdd.2009.44","creators":[{"id":"galluzzi_l","lastname":"Galluzzi"},{"id":"aaronson_s_a","lastname":"Aaronson"},{"id":"abrams_j","lastname":"Abrams"},{"id":"alnemri_e_s","lastname":"Alnemri"},{"id":"andrews_d_w","lastname":"Andrews"},{"id":"baehrecke_e_h","lastname":"Baehrecke"},{"id":"bazan_n_g","lastname":"Bazan"},{"id":"blagosklonny_m_v","lastname":"Blagosklonny"},{"id":"blomgren_k","lastname":"Blomgren"},{"id":"borner_c","lastname":"Borner"},{"id":"bredesen_d_e","lastname":"Bredesen"},{"id":"brenner_c","lastname":"Brenner"},{"id":"castedo_m","lastname":"Castedo"},{"id":"cidlowski_j_a","lastname":"Cidlowski"},{"id":"ciechanover_a","lastname":"Ciechanover"},{"id":"cohen_g_m","lastname":"Cohen"},{"id":"de_laurenzi_v","lastname":"De Laurenzi"},{"id":"de_maria_r","lastname":"De Maria"},{"id":"deshmukh_m","lastname":"Deshmukh"},{"id":"dynlacht_b_d","lastname":"Dynlacht"},{"id":"el_deiry_w_s","lastname":"El-Deiry"},{"id":"flavell_r_a","lastname":"Flavell"},{"id":"fulda_s","lastname":"Fulda"},{"id":"garrido_c","lastname":"Garrido"},{"id":"golstein_p","lastname":"Golstein"},{"id":"gougeon_m_l","lastname":"Gougeon"},{"id":"green_d_r","lastname":"Green"},{"id":"gronemeyer_h","lastname":"Gronemeyer"},{"id":"hajnoczky_g","lastname":"Hajn\u00f3czky"},{"id":"hardwick_j_m","lastname":"Hardwick"},{"id":"hengartner_m_o","lastname":"Hengartner"},{"id":"ichijo_h","lastname":"Ichijo"},{"id":"jaattela_m","lastname":"J\u00e4\u00e4ttel\u00e4"},{"id":"kepp_o","lastname":"Kepp"},{"id":"kimchi_a","lastname":"Kimchi"},{"id":"klionsky_d_j","lastname":"Klionsky"},{"id":"knight_r_a","lastname":"Knight"},{"id":"kornbluth_s","lastname":"Kornbluth"},{"id":"kumar_s","lastname":"Kumar"},{"id":"levine_b","lastname":"Levine"},{"id":"lipton_s_a","lastname":"Lipton"},{"id":"lugli_e","lastname":"Lugli"},{"id":"madeo_f","lastname":"Madeo"},{"id":"malomi_w","lastname":"Malomi"},{"id":"marine_j_c","lastname":"Marine"},{"id":"martin_s_j","lastname":"Martin"},{"id":"medema_j_p","lastname":"Medema"},{"id":"mehlen_p","lastname":"Mehlen"},{"id":"melino_g","lastname":"Melino"},{"id":"moll_u_m","lastname":"Moll"},{"id":"morselli_e","lastname":"Morselli"},{"id":"nagata_s","lastname":"Nagata"},{"id":"nicholson_d_w","lastname":"Nicholson"},{"id":"nicotera_p","lastname":"Nicotera"},{"id":"nunez_g","lastname":"Nu\u00f1ez"},{"id":"oren_m","lastname":"Oren"},{"id":"penninger_j","lastname":"Penninger"},{"id":"pervaiz_s","lastname":"Pervaiz"},{"id":"peter_m_e","lastname":"Peter"},{"id":"piacentini_m","lastname":"Piacentini"},{"id":"prehn_j_h","lastname":"Prehn"},{"id":"puthalakath_h","lastname":"Puthalakath"},{"id":"rabinovich_g_a","lastname":"Rabinovich"},{"id":"rizzuto_r","lastname":"Rizzuto"},{"id":"rodrigues_c_m","lastname":"Rodrigues"},{"id":"rubinsztein_d_c","lastname":"Rubinsztein"},{"id":"rudel_t","lastname":"Rudel"},{"id":"scorrano_l","lastname":"Scorrano"},{"id":"simon_h_u","lastname":"Simon"},{"id":"steller_h","lastname":"Steller"},{"id":"tschopp_j","lastname":"Tschopp"},{"id":"tsujimoto_y","lastname":"Tsujimoto"},{"id":"vandenabeele_p","lastname":"Vandenabeele"},{"id":"vitale_i","lastname":"Vitale"},{"id":"vousden_k_h","lastname":"Vousden"},{"id":"youle_r_j","lastname":"Youle"},{"id":"yuan_j","lastname":"Yuan"},{"id":"zhivotovsky_b","lastname":"Zhivotovsky"},{"id":"kroemer_g","lastname":"Kroemer"}]}

Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes.

Fiche du document

Mots-clés 0

Sujets proches En

Citer ce document

Métriques

Partage / Export

Résumé 0

Par les mêmes auteurs

Sur les mêmes sujets

Exporter en