Geneva cocktail for cytochrome p450 and P-glycoprotein activity assessment using dried blood spots.

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Auteurs
Date

2014

Type de document
Périmètre
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Identifiants
Source

Serveur académique Lausannois

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Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1038/clpt.2014.83

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/24722393

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1532-6535

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_94BFF516FD920

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Serveur Académique Lausannois

Organisation

Université de Lausanne et CHUV

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info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer

Mots-clés 0

Administration, Oral; Adult; Bupropion/administration & dosage; Bupropion/blood; Caffeine/administration & dosage; Caffeine/blood; Capsules; Carbonated Beverages; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Coffee; Cytochrome P-450 Enzyme System/antagonists & inhibitors; Cytochrome P-450 Enzyme System/blood; Dextromethorphan/administration & dosage; Dextromethorphan/blood; Dried Blood Spot Testing; Enzyme Inhibitors/administration & dosage; Feasibility Studies; Flurbiprofen/administration & dosage; Flurbiprofen/blood; Humans; Isoenzymes; Male; Midazolam/administration & dosage; Midazolam/blood; Omeprazole/administration & dosage; Omeprazole/blood; P-Glycoprotein/antagonists & inhibitors; P-Glycoprotein/blood; Pharmaceutical Preparations/administration & dosage; Pharmaceutical Preparations/blood; Phenotype; Pilot Projects; Predictive Value of Tests; Spectrometry, Mass, Electrospray Ionization; Substrate Specificity; Tandem Mass Spectrometry; Terfenadine/administration & dosage; Terfenadine/analogs & derivatives; Young Adult

Sujets proches En

Chromatography Analysis, Chromatographic

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M. Bosilkovska et al., « Geneva cocktail for cytochrome p450 and P-glycoprotein activity assessment using dried blood spots. », Serveur académique Lausannois, ID : 10.1038/clpt.2014.83

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The suitability of the capillary dried blood spot (DBS) sampling method was assessed for simultaneous phenotyping of cytochrome P450 (CYP) enzymes and P-glycoprotein (P-gp) using a cocktail approach. Ten volunteers received an oral cocktail capsule containing low doses of the probes bupropion (CYP2B6), flurbiprofen (CYP2C9), omeprazole (CYP2C19), dextromethorphan (CYP2D6), midazolam (CYP3A), and fexofenadine (P-gp) with coffee/Coke (CYP1A2) on four occasions. They received the cocktail alone (session 1), and with the CYP inhibitors fluvoxamine and voriconazole (session 2) and quinidine (session 3). In session 4, subjects received the cocktail after a 7-day pretreatment with the inducer rifampicin. The concentrations of probes/metabolites were determined in DBS and plasma using a single liquid chromatography-tandem mass spectrometry method. The pharmacokinetic profiles of the drugs were comparable in DBS and plasma. Important modulation of CYP and P-gp activities was observed in the presence of inhibitors and the inducer. Minimally invasive one- and three-point (at 2, 3, and 6 h) DBS-sampling methods were found to reliably reflect CYP and P-gp activities at each session.

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