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2015
- doi: 10.1038/ncomms7043
- issn: 2041-1723
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info:eu-repo/semantics/altIdentifier/pmid/25592181
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_F3B45145F27A2
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A. Larrieu et al., « A fluorescent hormone biosensor reveals the dynamics of jasmonate signalling in plants. », Serveur académique Lausannois, ID : 10.1038/ncomms7043
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Activated forms of jasmonic acid (JA) are central signals coordinating plant responses to stresses, yet tools to analyse their spatial and temporal distribution are lacking. Here we describe a JA perception biosensor termed Jas9-VENUS that allows the quantification of dynamic changes in JA distribution in response to stress with high spatiotemporal sensitivity. We show that Jas9-VENUS abundance is dependent on bioactive JA isoforms, the COI1 co-receptor, a functional Jas motif and proteasome activity. We demonstrate the utility of Jas9-VENUS to analyse responses to JA in planta at a cellular scale, both quantitatively and dynamically. This included using Jas9-VENUS to determine the cotyledon-to-root JA signal velocities on wounding, revealing two distinct phases of JA activity in the root. Our results demonstrate the value of developing quantitative sensors such as Jas9-VENUS to provide high-resolution spatiotemporal data about hormone distribution in response to plant abiotic and biotic stresses.