Fiche du document
- doi: 10.1038/s41396-022-01220-z
- issn: 1751-7362
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41396-022-01220-z
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/35338334
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1751-7370
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_B03B7173E5814
info:eu-repo/semantics/openAccess , CC BY 4.0 , https://creativecommons.org/licenses/by/4.0/
Sujets proches
Soil Mould, Vegetable Vegetable mold Earth (Soils) Mold, VegetableCiter ce document
K. Viacava et al., « Meta-omics-aided isolation of an elusive anaerobic arsenic-methylating soil bacterium. », Serveur académique Lausannois, ID : 10.1038/s41396-022-01220-z
Métriques
Partage / Export
Résumé
Soil microbiomes harbour unparalleled functional and phylogenetic diversity. However, extracting isolates with a targeted function from complex microbiomes is not straightforward, particularly if the associated phenotype does not lend itself to high-throughput screening. Here, we tackle the methylation of arsenic (As) in anoxic soils. As methylation was proposed to be catalysed by sulfate-reducing bacteria. However, to date, there are no available anaerobic isolates capable of As methylation, whether sulfate-reducing or otherwise. The isolation of such a microorganism has been thwarted by the fact that the anaerobic bacteria harbouring a functional arsenite S-adenosylmethionine methyltransferase (ArsM) tested to date did not methylate As in pure culture. Additionally, fortuitous As methylation can result from the release of non-specific methyltransferases upon lysis. Thus, we combined metagenomics, metatranscriptomics, and metaproteomics to identify the microorganisms actively methylating As in anoxic soil-derived microbial cultures. Based on the metagenome-assembled genomes of microorganisms expressing ArsM, we isolated Paraclostridium sp. strain EML, which was confirmed to actively methylate As anaerobically. This work is an example of the application of meta-omics to the isolation of elusive microorganisms.