CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions.
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12 février 2021
- doi: 10.1038/s41467-021-21366-2
- issn: 2041-1723
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info:eu-repo/semantics/altIdentifier/pmid/33579945
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_6E8BCA9FDAE91
info:eu-repo/semantics/openAccess , CC BY 4.0 , https://creativecommons.org/licenses/by/4.0/
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A. Kaushal et al., « CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions. », Serveur académique Lausannois, ID : 10.1038/s41467-021-21366-2
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Vertebrate genomes are partitioned into contact domains defined by enhanced internal contact frequency and formed by two principal mechanisms: compartmentalization of transcriptionally active and inactive domains, and stalling of chromosomal loop-extruding cohesin by CTCF bound at domain boundaries. While Drosophila has widespread contact domains and CTCF, it is currently unclear whether CTCF-dependent domains exist in flies. We genetically ablate CTCF in Drosophila and examine impacts on genome folding and transcriptional regulation in the central nervous system. We find that CTCF is required to form a small fraction of all domain boundaries, while critically controlling expression patterns of certain genes and supporting nervous system function. We also find that CTCF recruits the pervasive boundary-associated factor Cp190 to CTCF-occupied boundaries and co-regulates a subset of genes near boundaries together with Cp190. These results highlight a profound difference in CTCF-requirement for genome folding in flies and vertebrates, in which a large fraction of boundaries are CTCF-dependent and suggest that CTCF has played mutable roles in genome architecture and direct gene expression control during metazoan evolution.