Mechanistic insights into bacterial metabolic reprogramming from omics-integrated genome-scale models.
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2020
- doi: 10.1038/s41540-019-0121-4
- issn: 2056-7189
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info:eu-repo/semantics/altIdentifier/doi/10.1038/s41540-019-0121-4
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info:eu-repo/semantics/altIdentifier/pmid/31934348
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info:eu-repo/semantics/altIdentifier/eissn/2056-7189
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_AED3DDBE90054
info:eu-repo/semantics/openAccess , CC BY 4.0 , https://creativecommons.org/licenses/by/4.0/
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Environmental sciences; Systems analysisSujets proches
Primary metabolism Anabolism Metabolism, Primary CatabolismCiter ce document
N. Hadadi et al., « Mechanistic insights into bacterial metabolic reprogramming from omics-integrated genome-scale models. », Serveur académique Lausannois, ID : 10.1038/s41540-019-0121-4
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Understanding the adaptive responses of individual bacterial strains is crucial for microbiome engineering approaches that introduce new functionalities into complex microbiomes, such as xenobiotic compound metabolism for soil bioremediation. Adaptation requires metabolic reprogramming of the cell, which can be captured by multi-omics, but this data remains formidably challenging to interpret and predict. Here we present a new approach that combines genome-scale metabolic modeling with transcriptomics and exometabolomics, both of which are common tools for studying dynamic population behavior. As a realistic demonstration, we developed a genome-scale model of Pseudomonas veronii 1YdBTEX2, a candidate bioaugmentation agent for accelerated metabolism of mono-aromatic compounds in soil microbiomes, while simultaneously collecting experimental data of P. veronii metabolism during growth phase transitions. Predictions of the P. veronii growth rates and specific metabolic processes from the integrated model closely matched experimental observations. We conclude that integrative and network-based analysis can help build predictive models that accurately capture bacterial adaptation responses. Further development and testing of such models may considerably improve the successful establishment of bacterial inoculants in more complex systems.