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3 juillet 2018
- doi: 10.1038/s41598-018-27621-9
- issn: 2045-2322
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info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-27621-9
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info:eu-repo/semantics/altIdentifier/pmid/29968749
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info:eu-repo/semantics/altIdentifier/eissn/2045-2322
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_18BB59E932395
info:eu-repo/semantics/openAccess , CC BY 4.0 , https://creativecommons.org/licenses/by/4.0/
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H.M. Larsson et al., « Fiber density of collagen grafts impacts rabbit urethral regeneration. », Serveur académique Lausannois, ID : 10.1038/s41598-018-27621-9
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There is a need for efficient and "off-the-shelf" grafts in urethral reconstructive surgery. Currently available surgical techniques require harvesting of grafts from autologous sites, with increased risk of surgical complications and added patient discomfort. Therefore, a cost-effective and cell-free graft with adequate regenerative potential has a great chance to be translated into clinical practice. Tubular cell-free collagen grafts were prepared by varying the collagen density and fiber distribution, thereby creating a polarized low fiber density collagen graft (LD-graft). A uniform, high fiber density collagen graft (HD-graft) was engineered as a control. These two grafts were implanted to bridge a 2 cm long iatrogenic urethral defect in a rabbit model. Histology revealed that rabbits implanted with the LD-graft had a better smooth muscle regeneration compared to the HD-graft. The overall functional outcome assessed by contrast voiding cystourethrography showed patency of the urethra in 90% for the LD-graft and in 66.6% for the HD-graft. Functional regeneration of the rabbit implanted with the LD-graft could further be demonstrated by successful mating, resulting in healthy offspring. In conclusion, cell-free low-density polarized collagen grafts show better urethral regeneration than high-density collagen grafts.