Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant.
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2 octobre 2018
- doi: 10.1073/pnas.1807049115
- issn: 0027-8424
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info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.1807049115
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info:eu-repo/semantics/altIdentifier/pmid/30228123
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_3CB652F83F010
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C.T. Nguyen et al., « Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant. », Serveur académique Lausannois, ID : 10.1073/pnas.1807049115
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The identity of the cell files necessary for the leaf-to-leaf transmission of wound signals plants has been debated for decades. In Arabidopsis , wounding initiates the glutamate receptor-like (GLR)-dependent propagation of membrane depolarizations that lead to defense gene activation. Using a vein extraction procedure we found pools of GLR-fusion proteins in endomembranes in phloem sieve elements and/or in xylem contact cells. Strikingly, only double mutants that eliminated GLRs from both of these spatially separated cell types strongly attenuated leaf-to-leaf electrical signaling. glr3.3 mutants were also compromised in their defense against herbivores. Since wounding is known to cause increases in cytosolic calcium, we monitored electrical signals and Ca 2+ transients simultaneously. This revealed that wound-induced membrane depolarizations in the wild-type preceded cytosolic Ca 2+ maxima. The axial and radial distributions of calcium fluxes were differentially affected in each glr mutant. Resolving a debate over which cell types are necessary for electrical signaling between leaves, we show that phloem sieve elements and xylem contact cells function together in this process.