Transcription of the polyoma virus genome: synthesis and cleavage of giant late polyoma-specific RNA
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N. H. Acheson et al., « Transcription of the polyoma virus genome: synthesis and cleavage of giant late polyoma-specific RNA », Serveur académique Lausannois, ID : 10.1073/pnas.68.9.2231
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The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized "late" polyoma-specific RNA appears as "giant" molecules of heterogeneous size, up to several times larger than a strand of polyoma DNA (1.5 x 10(6) daltons). Treatment with dimethylsulfoxide or urea showed that the large size of these molecules is not due to aggregation. Giant polyoma-specific RNA is strikingly similar in size distribution to "nuclear messenger-like" RNA ("heterogeneous nuclear" RNA) of the host cell. Subsequent to its synthesis, some of the giant polyoma-specific RNA appears to be cleaved to at least three smaller species.