Demonstration of cross-reactive antibodies to smooth gram-negative bacteria in antiserum to Escherichia coli J5
Fiche du document
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1093/infdis/156.1.136
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/2439613
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pissn/0022-1899
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_6F758B9936FB2
info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer
Sujets proches
Immune globulins Antibodies Immune serum globulinCiter ce document
J. D. Baumgartner et al., « Demonstration of cross-reactive antibodies to smooth gram-negative bacteria in antiserum to Escherichia coli J5 », Serveur académique Lausannois, ID : 10.1093/infdis/156.1.136
Métriques
Partage / Export
Résumé
We investigated the discrepancy between the broad cross-protection against gram-negative infections afforded by antiserum to Escherichia coli J5 and its apparently narrow cross-reactivity in vitro. Rabbits immunized with J5 bacteria produced antibodies to both the J5 lipopolysaccharide (LPS; titer by ELISA, 1:60,000) and LPS from the Re mutant of Salmonella minnesota (i.e., to the ketodeoxyoctonate [KDO] and lipid A determinants; titer, 1:3,200). In highly diluted antiserum, titers of antibody to J5 LPS were reduced by 28%-41% after adsorption with seven strains of smooth gram-negative bacteria and by only 4% after adsorption with the Re mutant. Smooth gram-negative bacteria adsorbed virtually all antibody to Re LPS. Therefore, rabbit antiserum to J5 contains type-specific antibodies to core determinants distal to KDO that can obscure highly cross-reactive antibodies to lipid A-KDO in vitro. Cross-reactive antibodies are demonstrable by adsorption with whole bacteria at limiting concentrations of antibody.