Transcriptional analysis of left-sided colitis, pancolitis, and ulcerative colitis-associated dysplasia
Fiche du document
2014
- doi: 10.1097/MIB.0000000000000235
- issn: 1078-0998
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1097/MIB.0000000000000235
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/25358065
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1536-4844
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_52238F1DD46D8
info:eu-repo/semantics/restrictedAccess , Restricted: indefinite embargo , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer
Sujets proches
Chain reaction, Polymerase PCR (Biochemistry) Speech--Transcription Language and languages--Transcription Transcribing Immunohistology Expressive behavior Methods of analysis Analysis and chemistry Analytical methods Chemical analysis Analysis methods Analysis and examination TranscribingCiter ce document
J.T. Bjerrum et al., « Transcriptional analysis of left-sided colitis, pancolitis, and ulcerative colitis-associated dysplasia », Serveur académique Lausannois, ID : 10.1097/MIB.0000000000000235
Métriques
Partage / Export
Résumé
BACKGROUND: It is unknown why patients with extensive ulcerative colitis (UC) have a higher risk of colorectal cancer compared with patients with left-sided UC. This study characterizes the inflammatory processes in left-sided UC, pancolitis, and UC-associated dysplasia at the transcriptional level to identify potential biomarkers and transcripts of importance for the carcinogenic behavior of chronic inflammation. METHODS: The Affymetrix GeneChip Human Genome U133 Plus 2.0 was applied on colonic biopsies from UC patients with left-sided UC, pancolitis, dysplasia, and controls. Reverse transcription polymerase chain reaction and immunohistochemistry were performed for validating selected transcripts in the initial cohort and in 2 independent cohorts of patients with UC. Microarray data were analyzed by principal component analysis, and reverse transcription polymerase chain reaction and immunohistochemistry data by the Wilcoxon's rank-sum test. RESULTS: The principal component analysis results revealed separate clusters for left-sided UC, pancolitis, dysplasia, and controls. Close clustering of dysplastic and pancolitic samples indicated similarities in gene expression. Indeed, 101 and 656 parallel upregulated and downregulated transcripts, respectively, were identified in specimens from dysplasia and pancolitis. Validation of selected transcripts hereof identified insulin receptor alpha (INSRA) and MAP kinase interacting serine/threonine kinase 2 (MKNK2) with an enhanced expression in dysplasia compared with left-sided UC and controls, whereas laminin γ2 (LAMC2) was found with a lower expression in dysplasia compared with the remaining 3 groups. CONCLUSIONS: This study demonstrates pancolitis and left-sided UC as distinct inflammatory processes at the transcriptional level, and identifies INSRA, MKNK2, and LAMC2 as potential critical transcripts in the inflammation-driven preneoplastic process of UC.