A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system.
Fiche du document
2013
- doi: 10.1099/jmm.0.049361-0
- issn: 0022-2615
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.049361-0
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/23329320
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1473-5644
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_ACF3DD1B42CD2
info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer
Sujets proches
Enteric bacteria Colon bacilli Fecal coliforms Fecal coliform bacteria Enterics (Bacteria) Enterobacteria Coliform bacilli Coliforms Coliform bacteria Enteric bacilliCiter ce document
Prod'hom G. et al., « A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system. », Serveur académique Lausannois, ID : 10.1099/jmm.0.049361-0
Métriques
Partage / Export
Résumé
An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3% for Enterobacteriaceae, and 0.7 and 0.1% for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci.