2014
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1111/ejn.12481
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/24443946
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1460-9568
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_7CEF4F60D81A5
info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer
J.S. Ignatious Raja et al., « Role of Go/i subgroup of G proteins in olfactory signaling of Drosophila melanogaster. », Serveur académique Lausannois, ID : 10.1111/ejn.12481
Intracellular signaling in insect olfactory receptor neurons remains unclear, with both metabotropic and ionotropic components being discussed. Here, we investigated the role of heterotrimeric Go and Gi proteins using a combined behavioral, in vivo and in vitro approach. Specifically, we show that inhibiting Go in sensory neurons by pertussis toxin leads to behavioral deficits. We heterologously expressed the olfactory receptor dOr22a in human embryonic kidney cells (HEK293T). Stimulation with an odor led to calcium influx, which was amplified via calcium release from intracellular stores. Subsequent experiments indicated that the signaling was mediated by the Gβγ subunits of the heterotrimeric Go/i proteins. Finally, using in vivo calcium imaging, we show that Go and Gi contribute to odor responses both for the fast (phasic) as for the slow (tonic) response component. We propose a transduction cascade model involving several parallel processes, in which the metabotropic component is activated by Go and Gi , and uses Gβγ.