Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2000.tb09340.x
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/11024264
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pissn/0378-1097
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_EEBF69E055C21
info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer
C. Mueller et al., « A mutagenic PCR identifies isolates of Borrelia garinii responsible for Lyme borreliosis », Serveur académique Lausannois, ID : 10.1111/j.1574-6968.2000.tb09340.x
Borrelia garinii is one of the three major Borreliae responsible for Lyme borreliosis in Europe. We have characterized a protein of B. garinii (VS102) and a genomic fragment from the gene encoding this protein was cloned. The DNA sequence of the fragment showed high homology with a known gene of B. burgdorferi sensu stricto. The protein encoded by this gene in B. burgdorferi sensu stricto is a phosphocarrier protein (histidine-containing protein). A mutation T to G polymorphism at codon 57 was found to be specific to B. garinii. A PCR-based approach that allows the rapid detection of this mutation made it possible to specifically discriminate B. garinii from other B. burgdorferi genospecies with high sensitivity and specificity.