17 septembre 2021
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1126/sciadv.abg6718
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/34533984
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/2375-2548
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_3987E09FB9740
info:eu-repo/semantics/openAccess , CC BY-NC 4.0 , https://creativecommons.org/licenses/by-nc/4.0/
V. Gerganova et al., « Cell patterning by secretion-induced plasma membrane flows. », Serveur académique Lausannois, ID : 10.1126/sciadv.abg6718
Cells self-organize using reaction-diffusion and fluid-flow principles. Whether bulk membrane flows contribute to cell patterning has not been established. Here, using mathematical modeling, optogenetics, and synthetic probes, we show that polarized exocytosis causes lateral membrane flows away from regions of membrane insertion. Plasma membrane-associated proteins with sufficiently low diffusion and/or detachment rates couple to the flows and deplete from areas of exocytosis. In rod-shaped fission yeast cells, zones of Cdc42 GTPase activity driving polarized exocytosis are limited by GTPase activating proteins (GAPs). We show that membrane flows pattern the GAP Rga4 distribution and that coupling of a synthetic GAP to membrane flows is sufficient to establish the rod shape. Thus, membrane flows induced by Cdc42-dependent exocytosis form a negative feedback restricting the zone of Cdc42 activity.