Selective in vivo visualization of immune-cell infiltration in a mouse model of autoimmune myocarditis by fluorine-19 cardiac magnetic resonance.

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Date

2013

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Périmètre
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Serveur académique Lausannois

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Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1161/CIRCIMAGING.112.000125

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/23343515

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1942-0080

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_C7B2751B340B5

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Serveur Académique Lausannois

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Université de Lausanne et CHUV

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info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer

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Animals; Autoimmune Diseases/immunology; Autoimmune Diseases/metabolism; Dendritic Cells/immunology; Disease Models, Animal; Flow Cytometry; Fluorine/administration & dosage; Fluorine/diagnostic use; Fluorocarbons/administration & dosage; Fluorocarbons/diagnostic use; Granulocytes/immunology; Immunohistochemistry; Injections, Intravenous; Lymphocytes/immunology; Macrophages/immunology; Magnetic Resonance Imaging; Male; Mice; Mice, Inbred BALB C; Myeloid Cells/immunology; Myeloid Cells/metabolism; Myocarditis/immunology; Myocarditis/metabolism; Myocardium/immunology; Myocardium/metabolism; Predictive Value of Tests; Severity of Illness Index; Signal-To-Noise Ratio

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Myocardium--Inflammation Mouse House mice Mus musculus House mouse

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R.B. van Heeswijk et al., « Selective in vivo visualization of immune-cell infiltration in a mouse model of autoimmune myocarditis by fluorine-19 cardiac magnetic resonance. », Serveur académique Lausannois, ID : 10.1161/CIRCIMAGING.112.000125

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BACKGROUND: The goal of this study was to characterize the performance of fluorine-19 ((19)F) cardiac magnetic resonance (CMR) for the specific detection of inflammatory cells in a mouse model of myocarditis. Intravenously administered perfluorocarbons are taken up by infiltrating inflammatory cells and can be detected by (19)F-CMR. (19)F-labeled cells should, therefore, generate an exclusive signal at the inflamed regions within the myocardium. METHODS AND RESULTS: Experimental autoimmune myocarditis was induced in BALB/c mice. After intravenous injection of 2×200 µL of a perfluorocarbon on day 19 and 20 (n=9) after immunization, in vivo (19)F-CMR was performed at the peak of myocardial inflammation (day 21). In 5 additional animals, perfluorocarbon combined with FITC (fluorescein isothiocyanate) was administered for postmortem immunofluorescence and flow-cytometry analyses. Control experiments were performed in 9 animals. In vivo (19)F-CMR detected myocardial inflammation in all experimental autoimmune myocarditis-positive animals. Its resolution was sufficient to identify even small inflammatory foci, that is, at the surface of the right ventricle. Postmortem immunohistochemistry and flow cytometry confirmed the presence of perfluorocarbon in macrophages, dendritic cells, and granulocytes, but not in lymphocytes. The myocardial volume of elevated (19)F signal (rs=0.96; P

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