Fiche du document
2006
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2164-7-176
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/16836751
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pissn/1471-2164
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_AB5ACB7D97C21
info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer
Sujets proches
Template RNA Informational RNA Protein transcript Messenger ribonucleic acid mRNA Protein transcriptsCiter ce document
D. Retelska et al., « Similarities and differences of polyadenylation signals in human and fly. », Serveur académique Lausannois, ID : 10.1186/1471-2164-7-176
Métriques
Partage / Export
Résumé
BACKGROUND: Cleavage of messenger RNA (mRNA) precursors is an essential step in mRNA maturation. The signal recognized by the cleavage enzyme complex has been characterized as an A rich region upstream of the cleavage site containing a motif with consensus AAUAAA, followed by a U or UG rich region downstream of the cleavage site. RESULTS: We studied these signals using exhaustive databases of cleavage sites obtained from aligning raw expressed sequence tags (EST) sequences to genomic sequences in Homo sapiens and Drosophila melanogaster. These data show that the polyadenylation signal is highly conserved in human and fly. In addition, de novo motif searches generated a refined description of the U-rich downstream sequence (DSE) element, which shows more divergence between the two species. These refined motifs are applied, within a Hidden Markov Model (HMM) framework, to predict mRNA cleavage sites. CONCLUSION: We demonstrate that the DSE is a specific motif in both human and Drosophila. These findings shed light on the sequence correlates of a highly conserved biological process, and improve in silico prediction of 3' mRNA cleavage and polyadenylation sites.