A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.

M.M. Simon; S. Greenaway; J.K. White; H. Fuchs; V. Gailus-Durner; S. Wells; T. Sorg; K. Wong; E. Bedu; E.J. Cartwright; R. Dacquin; S. Djebali; J. Estabel; J. Graw; N.J. Ingham; I.J. Jackson; A. Lengeling; S. Mandillo; J. Marvel; H. Meziane; F. Preitner; O. Puk; M. Roux; D.J. Adams; S. Atkins; A. Ayadi; L. Becker; A. Blake; D. Brooker; H. Cater; M.F. Champy; R. Combe; P. Danecek; A. di Fenza; H. Gates; A.K. Gerdin; E. Golini; J.M. Hancock; W. Hans; S.M. Hölter; T. Hough; P. Jurdic; T.M. Keane; H. Morgan; W. Müller; F. Neff; G. Nicholson; B. Pasche; L.A. Roberson; J. Rozman; M. Sanderson; L. Santos; M. Selloum; C. Shannon; A. Southwell; G.P. Tocchini-Valentini; V.E. Vancollie; H. Westerberg; W. Wurst; M. Zi; B. Yalcin; R. Ramirez-Solis; K.P. Steel; A.M. Mallon; M.H. de Angelis; Y. Herault; S.D. Brown

A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.

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Date

2013

Discipline

Sciences de l'information et de la communication

Type de document

Articles

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Publications

Langue

Anglais

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doi: 10.1186/gb-2013-14-7-r82
issn: 1465-6906

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Serveur académique Lausannois

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Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1186/gb-2013-14-7-r82

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/23902802

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/eissn/1465-6914

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_90419D2019AD0

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Serveur Académique Lausannois

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Université de Lausanne et CHUV

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info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer

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Mouse inbred lines; sequence variation; mouse phenotyping; gene knockout; C57BL/6

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Mouse House mice Mus musculus House mouse

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M.M. Simon et al., « A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains. », Serveur académique Lausannois, ID : 10.1186/gb-2013-14-7-r82

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BACKGROUND: The mouse inbred line C57BL/6J is widely used in mouse genetics and its genome has been incorporated into many genetic reference populations. More recently large initiatives such as the International Knockout Mouse Consortium (IKMC) are using the C57BL/6N mouse strain to generate null alleles for all mouse genes. Hence both strains are now widely used in mouse genetics studies. Here we perform a comprehensive genomic and phenotypic analysis of the two strains to identify differences that may influence their underlying genetic mechanisms. RESULTS: We undertake genome sequence comparisons of C57BL/6J and C57BL/6N to identify SNPs, indels and structural variants, with a focus on identifying all coding variants. We annotate 34 SNPs and 2 indels that distinguish C57BL/6J and C57BL/6N coding sequences, as well as 15 structural variants that overlap a gene. In parallel we assess the comparative phenotypes of the two inbred lines utilizing the EMPReSSslim phenotyping pipeline, a broad based assessment encompassing diverse biological systems. We perform additional secondary phenotyping assessments to explore other phenotype domains and to elaborate phenotype differences identified in the primary assessment. We uncover significant phenotypic differences between the two lines, replicated across multiple centers, in a number of physiological, biochemical and behavioral systems. CONCLUSIONS: Comparison of C57BL/6J and C57BL/6N demonstrates a range of phenotypic differences that have the potential to impact upon penetrance and expressivity of mutational effects in these strains. Moreover, the sequence variants we identify provide a set of candidate genes for the phenotypic differences observed between the two strains.

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A comparative phenotypic and genomic analysis of C57BL/6J and C57BL/6N mouse strains.

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