Distinct nitrogen isotopic compositions of healthy and cancerous tissue in mice brain and head&neck micro-biopsies.
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13 juillet 2021
- doi: 10.1186/s12885-021-08489-x
- issn: 1471-2407
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info:eu-repo/semantics/altIdentifier/doi/10.1186/s12885-021-08489-x
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info:eu-repo/semantics/altIdentifier/pmid/34256713
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_81B033323C181
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M. Straub et al., « Distinct nitrogen isotopic compositions of healthy and cancerous tissue in mice brain and head&neck micro-biopsies. », Serveur académique Lausannois, ID : 10.1186/s12885-021-08489-x
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Cancerous cells can recycle metabolic ammonium for their growth. As this ammonium has a low nitrogen isotope ratio ( 15 N/ 14 N), its recycling may cause cancer tissue to have lower 15 N/ 14 N than surrounding healthy tissue. We investigated whether, within a given tissue type in individual mice, tumoral and healthy tissues could be distinguished based on their 15 N/ 14 N. Micro-biopsies of murine tumors and adjacent tissues were analyzed for 15 N/ 14 N using novel high-sensitivity methods. Isotopic analysis was pursued in Nude and C57BL/6 mice models with mature orthotopic brain and head&neck tumors generated by implantation of H454 and MEERL95 murine cells, respectively. In the 7 mice analyzed, the brain tumors had distinctly lower 15 N/ 14 N than healthy neural tissue. In the 5 mice with head&neck tumors, the difference was smaller and more variable. This was at least partly due to infiltration of healthy head&neck tissue by tumor cells. However, it may also indicate that the 15 N/ 14 N difference between tumoral and healthy tissue depends on the nitrogen metabolism of the healthy organ in question. The findings, coupled with the high sensitivity of the 15 N/ 14 N measurement method used here, suggest a new approach for micro-biopsy-based diagnosis of malignancy as well as an avenue for investigation of cancer metabolism.