FlyLimbTracker: An active contour based approach for leg segment tracking in unmarked, freely behaving Drosophila.
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2017
- doi: 10.1371/journal.pone.0173433
- issn: 1932-6203
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Pelvic limbs Lower limb Extremities, Lower Pelvic limb Limb, Lower Extremity, Lower Legs Limb, Pelvic Lower extremities Lower extremity Lower limbsCiter ce document
V. Uhlmann et al., « FlyLimbTracker: An active contour based approach for leg segment tracking in unmarked, freely behaving Drosophila. », Serveur académique Lausannois, ID : 10.1371/journal.pone.0173433
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Résumé
Understanding the biological underpinnings of movement and action requires the development of tools for quantitative measurements of animal behavior. Drosophila melanogaster provides an ideal model for developing such tools: the fly has unparalleled genetic accessibility and depends on a relatively compact nervous system to generate sophisticated limbed behaviors including walking, reaching, grooming, courtship, and boxing. Here we describe a method that uses active contours to semi-automatically track body and leg segments from video image sequences of unmarked, freely behaving D. melanogaster. We show that this approach yields a more than 6-fold reduction in user intervention when compared with fully manual annotation and can be used to annotate videos with low spatial or temporal resolution for a variety of locomotor and grooming behaviors. FlyLimbTracker, the software implementation of this method, is open-source and our approach is generalizable. This opens up the possibility of tracking leg movements in other species by modifications of underlying active contour models.