Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.
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2012
- doi: 10.3109/15419061.2012.690792
- issn: 1543-5180
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info:eu-repo/semantics/altIdentifier/doi/10.3109/15419061.2012.690792
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info:eu-repo/semantics/altIdentifier/pmid/22642233
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_A71D20B17C239
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N. Halidi et al., « Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43. », Serveur académique Lausannois, ID : 10.3109/15419061.2012.690792
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Résumé
Intercellular Ca(2+) wave propagation between vascular smooth muscle cells (SMCs) is associated with the propagation of contraction along the vessel. Here, we characterize the involvement of gap junctions (GJs) in Ca(2+) wave propagation between SMCs at the cellular level. Gap junctional communication was assessed by the propagation of intercellular Ca(2+) waves and the transfer of Lucifer Yellow in A7r5 cells, primary rat mesenteric SMCs (pSMCs), and 6B5N cells, a clone of A7r5 cells expressing higher connexin43 (Cx43) to Cx40 ratio. Mechanical stimulation induced an intracellular Ca(2+) wave in pSMC and 6B5N cells that propagated to neighboring cells, whereas Ca(2+) waves in A7r5 cells failed to progress to neighboring cells. We demonstrate that Cx43 forms the functional GJs that are involved in mediating intercellular Ca(2+) waves and that co-expression of Cx40 with Cx43, depending on their expression ratio, may interfere with Cx43 GJ formation, thus altering junctional communication.