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15 octobre 2019
- doi: 10.3390/ijms20205115
- issn: 1422-0067
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_9AE31044F6EF6
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J. Chen et al., « The Potential of the FSP1cre-Pparb/d-/- Mouse Model for Studying Juvenile NAFLD. », Serveur académique Lausannois, ID : 10.3390/ijms20205115
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Non-alcoholic fatty liver disease (NAFLD) can progress from steatosis to non-alcoholic steatohepatitis (NASH) characterized by liver inflammation, possibly leading to cirrhosis and hepatocellular carcinoma (HCC). Mice with impaired macrophage activation, when fed a high-fat diet, develop severe NASH. Evidence is mounting that Kupffer cells are implicated. However, it is unknown whether the resident CD68 + or bone marrow-derived CD11b + Kupffer cells are involved. Characterization of the FSP1cre-Pparb/d - / - mouse liver revealed that FSP1 is expressed in CD11b + Kupffer cells. Although these cells only constitute a minute fraction of the liver cell population, Pparb/d deletion in these cells led to remarkable hepatic phenotypic changes. We report that a higher lipid content was present in postnatal day 2 (P2) FSP1cre-Pparb/d - / - livers, which diminished after weaning. Quantification of total lipids and triglycerides revealed that P2 and week 4 of age FSP1cre-Pparb/d -/- livers have higher levels of both. qPCR analysis also showed upregulation of genes involved in fatty acid β-oxidation, and fatty acid and triglyceride synthesis pathways. This result is further supported by western blot analysis of proteins in these pathways. Hence, we propose that FSP1cre-Pparb/d -/- mice, which accumulate lipids in their liver in early life, may represent a useful animal model to study juvenile NAFLD.
