Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment.
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8 novembre 2022
- doi: 10.3390/life12111822
- issn: 2075-1729
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info:eu-repo/semantics/altIdentifier/doi/10.3390/life12111822
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info:eu-repo/semantics/altIdentifier/pmid/36362977
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info:eu-repo/semantics/altIdentifier/pissn/2075-1729
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info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_38524C9AF7BA0
info:eu-repo/semantics/openAccess , CC BY 4.0 , https://creativecommons.org/licenses/by/4.0/
Mots-clés
Arabidopsis thaliana; RNA sequencing; glucosinolates; nitriles; non-thermal plasma; plant defenseSujets proches
Cress, Mouseear Sisymbrium thalianum Arabis thaliana Thale cress Cress, Mouse-ear Cress, Thale Mouseear cress Mouse-ear cress Plants--Seeds Crops--Seeds Ribose nucleic acid Ribonucleic acid Days of the week SeedCiter ce document
A. Waskow et al., « Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment. », Serveur académique Lausannois, ID : 10.3390/life12111822
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Résumé
Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old Arabidopsis thaliana (L.) Heynh. seedlings grown from surface dielectric barrier discharge plasma-treated seeds. In a previous study, seeds were analyzed 6 days after plasma exposure and a plant stress and defense response was observed. Here, we performed a pathway analysis on differentially expressed genes and our results revealed again an increased expression of plant stress and defense, specifically glucosinolate pathway-related compounds. The main difference was that a different part of the plant defense response changed at 7 days, which was not previously observed at 6 days. With a 24-h delayed extraction time point, the glucosinolates were selectively broken down into nitriles among all of the glucosinolates catabolic products. Although information about nitriles is limited, it protects plants against biotic stresses and has variable toxicity depending on the interacting organism. More work needs to be performed to better understand which plasma seed treatment parameters affect plant defense; however, these preliminary findings suggest that an optimized plasma treatment could be used to elicit a plant defense response.