Fiche du document
29 juillet 2017
- doi: 10.3390/nano7080202
- issn: 2079-4991
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.3390/nano7080202
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/28758913
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pissn/2079-4991
Ce document est lié à :
info:eu-repo/semantics/altIdentifier/urn/urn:nbn:ch:serval-BIB_BE46CDFFBA657
info:eu-repo/semantics/openAccess , Copying allowed only for non-profit organizations , https://serval.unil.ch/disclaimer
Sujets proches
Red iron trioxide Ferric trioxide Iron oxide Red ferric oxide Red iron oxide Ironic oxideCiter ce document
D. Bonvin et al., « Versatility of Pyridoxal Phosphate as a Coating of Iron Oxide Nanoparticles. », Serveur académique Lausannois, ID : 10.3390/nano7080202
Métriques
Partage / Export
Résumé
Pyridoxal 5'-phosphate (PLP) is the most important cofactor of vitamin B₆-dependent enzymes, which catalyses a wide range of essential body functions (e.g., metabolism) that could be exploited to specifically target highly metabolic cells, such as tumour metastatic cells. However, the use of PLP as a simultaneous coating and targeting molecule, which at once provides colloidal stability and specific biological effects has not been exploited so far. Therefore, in this work iron oxide nanoparticles (IONPs) were coated by PLP at two different pH values to tune PLP bonding (e.g., orientation) at the IONP surface. The surface study, as well as calculations, confirmed different PLP bonding to the IONP surface at these two pH values. Moreover, the obtained PLP-IONPs showed different zeta potential, hydrodynamic radius and agglomeration state, and consequently different uptake by two metastatic-prostate-cancer cell lines (LnCaP and PC3). In LnCaP cells, PLP modified the morphology of IONP-containing intracellular vesicles, while in PC3 cells PLP impacted the amount of IONPs taken up by cells. Moreover, PLP-IONPs displayed high magnetic resonance imaging (MRI) r₂ relaxivity and were not toxic for the two studied cell lines, rendering PLP promising for biomedical applications. We here report the use of PLP simultaneously as a coating and targeting molecule, directly bound to the IONP surface, with the additional high potential for MRI detection.