Detection of functional antigen-specific T cells from urine of non-muscle invasive bladder cancer patients.
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2012
- doi: 10.4161/onci.20526
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Pee Thymus-dependent lymphocytes Thymus-derived cells T lymphocytes Thymus-dependent cellsCiter ce document
C. Pieraerts et al., « Detection of functional antigen-specific T cells from urine of non-muscle invasive bladder cancer patients. », Serveur académique Lausannois, ID : 10.4161/onci.20526
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To gain further insights into the role of T lymphocytes in immune responses against bladder tumors, we developed a method that monitors the presence of functional antigen-specific T cells in the urine of non-muscle invasive bladder cancer patients. As relatively few immune cells can usually be recovered from urine, we examined different isolation/amplification protocols and took advantage of patients treated with weekly intravesical instillations of Bacillus Calmette-Guérin, resulting in large amounts of immune cells into urine. Our findings demonstrate that, upon in vitro amplification, antigen-specific T cells can be detected by an interferon γ (IFNγ)-specific ELISPOT assay.