Time-Course RNA-Seq analysis of Arabidopsis thaliana shoot meristems during floral transition (accession Col-0)

Résumé 0

Time-course RNA sequencing during floral transition of micro-dissected meristems from the main shoot of the model plant Arabidopsis thaliana. Plants are wild-type of the reference accession Col-0, (named C in the datasets), derived from stock 186AV of the Versailles Arabidopsis Stock Center. The time-course is made of four sampling time points corresponding to four developmental stages assessed from temporal and phenotypical cues observed under a binocular on the plant meristems: Vegetative (V), Late vegetative (L), Transitioning (T) and Floral (F). Five to ten micro-dissected meristems were pooled per time point and the entire time course was replicated three times independantly (see Dieudonné S. et al. for details). cDNA was sequenced using NextSeq500 from Illumina. After classical read quality filtering, each sample yielded on average 43 million sequences.