Dataset concerning zootechnical traits, biological indicators, oocyte collection and embryo production from ewes with contrasted metabolic status and submitted to chronic Bisphenol S exposure.

Résumé 0

This dataset consists in raw data from an experimental design in the ovine model: zootechnical traits, biological indicators, oocyte collection and embryo production from ewes with contrasted metabolic status and submitted to chronic Bisphenol S exposure. During 2 consecutive years, 120 primiparous ewes (60 ewes/year), were managed in a loose sheepfold, assigned to two diet groups - Restricted (R, n = 30) and Well-fed (WF, n = 30) - and supplemented with or without BPS (0, 4 or 50 μg/kg/day), generating 6 experimental groups named R-0, R-4, R-50, WF-0, WF-4, and WF-50 (n=10 ewes/group). Oocyte collection was carried out during the breeding season of sheep between September and December. Diet management started in May and BPS treatment started in June, that is to say at least 3 months before oocyte collection. The dataset presents zootechnical traits (age, body weight, body condition score), metabolic indicators and hormones concentrations measured in plasma (non-esterified fatty acids (NEFA), glucose, thyroid hormones, Anti-Mullerian hormone (AMH), progesterone, oestradiol and others steroids) as well as in follicular fluid for some of them (progesterone, oestradiol and others steroids), Bisphenol S (BPS) and BPS-glucuronide concentrations in plasma and urine, data from follicular puncture and oocyte collection by ovum pick-up followed by in vitro embryo production (during the 2nd year of experiment). All experimental procedures were conducted in accordance with the European Directive 2010/63/EU on the protection of animals used for scientific purposes and approved by the French Ministry of National Education, Higher Education, Research and Innovation after ethical assessment by the local ethics committee “Comité d’Ethique en Expérimentation Animale Val de Loire (CEEA VdL)” (protocols registered under APAFIS numbers 13965–2018042008519239v2 and 14014–2018030717477406v2). This large dataset can be re-used on the one hand by researchers working on reproductive physiology in interaction with energy metabolism in ruminants, to further investigate the effects a restricted diet; on the other hand, by toxicologists and endocrinologists to investigate the effects of bisphenol S (potential endocrine disruptor) on several hormones (i.e. steroids) and metabolites.