The use of nanopore sequencing to analyze the chloroplast transcriptome part 2 – bioinformatic analyzes and virtual RN blots
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6 juin 2024
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- doi: 10.57745/UWAHNR
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Agricultural SciencesSujets proches
Generation Procreation Pangenesis AmphimixisCiter ce document
Etienne DELANNOY, « The use of nanopore sequencing to analyze the chloroplast transcriptome part 2 – bioinformatic analyzes and virtual RN blots », Recherche Data Gouv, ID : 10.57745/UWAHNR
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Nanopore sequencing of full-length cDNAs offers unprecedented details of the plastid RNA metabolism. After the generation of the nanopore reads, several bioinformatic steps are required to analyze the data. In this chapter, we describe in a few simple command lines the processing and mapping of the reads as well as the generation of virtual Northern blots as a simple and familiar way to visualize Nanopore sequencing data.