Genomic DNA sequencing from a bulk segregant experiment to map the genetic determinants of natural bracts variation in Arabidopsis thaliana

Résumé 0

Paired-end Illumina sequences of genomic DNA used to map the genetic variant responsible for bract development in the wild-type accession Tsu-0 of the model plant Arabidopsis thaliana. Bract presence was quantified by a bract score. Crosses in both directions were done between the wild accession Tsu-0 (obtained from Versailles Arabidopsis Stock center -VASC- , stock 91AV) showing a high bract score and the reference accession Col-0 (VASC stock 186AV) displaying a low bract score. Two bulks of F2 plants were selected, with a high (Tsu-0 like) and low (Col-0 like) bract score, respectively. Genomic DNA of each plants of the bulks was extracted and mixed in equal quantities. The four samples therefore correspond to genomic DNA of the two parents (Tsu-0 and Col-0) and of the two bulks (low and high bract score). DNA concentrations: Col-0 = 25,6ng/µl (a few plants) ; Tsu-0 (a few plants) = 15,5ng/µl, high bract score F2 bulk (17 plants): 13,2ng/µl ; low bract score F2 bulk (56 plants): 14,6ng/µl Genomic DNA was extracted using a CTAB protocol and sequenced with Illumina-like technology by BGI (BGISEQ) with 100 base pair paired end reads, yielding 5G data for each sample (~ 40X).