Effect of seasonal infertility period on boar sperm proteins and quality characteristics Effect of seasonal infertility period on boar sperm proteins and quality characteristics El En

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27 janvier 2020

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eJournals

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https://ejournals.epublishing.ekt.gr/index.php/jhv [...]

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Copyright (c) 2020 I.A. MICHOS, I.A. TSAKMAKIDIS, M. TSANTARLIOTOU, G. TSOUSIS, P. HADWEH, C.M. BOSCOS , https://creativecommons.org/licenses/by-nc/4.0

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boar semen semen analysis HSP90 GPX5 seasonal infertility boar semen semen analysis HSP90 GPX5 seasonal infertility

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Sperm Male gametes

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I.A. MICHOS et al., « Effect of seasonal infertility period on boar sperm proteins and quality characteristics », eJournals, ID : 10670/1.4wfn3j

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ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗ

Swine seasonal infertility reduces the productivity and profitability of a pig farm. The main causes of this condition are elevated environmental temperatures and long photoperiod during the summer season. The aim of this study was to investigate which sperm proteins and parameters are affected during the period of seasonal infertility. Depending on the environmental temperatures, the period from October to June was considered as cold and the period from July to September as warm season. A total of 65 ejaculates from 18 boars were collected over a year. Each semen sample was evaluated for kinetics (Computer Assisted Semen Analyzer), morphology (Sperm Blue stain), viability (Propidium Iodide - Calcein AM stain), mitochondrial membrane potential (Rhodamine 123 – Propidium Iodide stain), membrane integrity and functionality (Hypo-osmotic swelling test) and sperm DNA integrity (Acridine Orange Test). Moreover, selected proteins (HSP90, GPX5, OPN) were detected and quantified. The kinetic parameters VSL, LIN and the midpiece abnormalities were significantly higher in the warm compared to the cold season (p

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