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1 janvier 2018
- handle: 10670/1.6m60q1
- issn: 1945-0494
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R.O. Eren et al., « Development of a semi-automated image-based high-throughput drug screening system. », Serveur académique Lausannois, ID : 10670/1.6m60q1
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We previously reported that the innate sensing of the endosymbiont Leishmania RNA virus 1 (LRV1) within Leishmania (Viannia) guyanensis through Toll-like receptor 3, worsens the pathogenesis of parasite infection in mice. The presence of LRV1 has been associated with the failure of first-line treatment in patients infected with LRV1 containing - L. guyanensis and - L. braziliensis parasites. Here, we established a semi-automated image-based high-throughput drug screening (HTDS) protocol to measure parasiticidal activity of the Prestwick chemical library in primary murine macrophages infected with LRV1-containing L. guyanensis . The two-independent screens generated 14 hit compounds with over sixty-nine percent reduction in parasite growth compared to control, at a single dose in both screens. Our screening strategy offers great potential in the search for new drugs and accelerates the discovery rate in the field of drug repurposing against Leishmania . Moreover, this technique allows the concomitant assessment of the effect of drug toxicity on host cell number.