The millennial dynamics of malaria in the mediterranean basin: documenting Plasmodium spp. on the medieval island of Corsica
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2023
- ISIDORE Id: 10670/1.8169c8...
- halshs: halshs-04333056
- doi: 10.3389/fmed.2023.1265964
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info:eu-repo/semantics/altIdentifier/doi/10.3389/fmed.2023.1265964
info:eu-repo/semantics/OpenAccess
Mots-clés
Plasmodium falciparum Plasmodium ovale ancient malaria Corsica paleo-auto-immunohistochemistry metagenomic immunochromatography Paludisme Moyen âge Méditerranée archéologie -bio]/Human health and pathology/Infectious diseases -bio]/Microbiology and Parasitology/ParasitologySujets proches
Malaria parasitesCiter ce document
Mahmoud Abdelwadoud Boualam et al., « The millennial dynamics of malaria in the mediterranean basin: documenting Plasmodium spp. on the medieval island of Corsica », HAL SHS (Sciences de l’Homme et de la Société), ID : 10.3389/fmed.2023.1265964
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Introduction: The lack of well-preserved material upon which to base the paleo-microbiological detection of Plasmodium parasites has prevented extensive documentation of past outbreaks of malaria in Europe. By trapping intact erythrocytes at the time of death, dental pulp has been shown to be a suitable tissue for documenting ancient intraerythrocytic pathogens such as Plasmodium parasites.Methods: Total DNA and proteins extracted from 23 dental pulp specimens collected from individuals exhumed from the 9th to 13th century archaeological site in Mariana, Corsica, were analyzed using open-mind paleo-auto-immunohistochemistry and direct metagenomics, Plasmodium -targeting immunochromatography assays. All experiments incorporated appropriate negative controls.Results: Paleo-auto-immunohistochemistry revealed the presence of parasites Plasmodium spp. in the dental pulp of nine teeth. A further immunochromatography assay identified the presence of at least one Plasmodium antigen in nine individuals. The nine teeth, for which the PfHRP-2 antigen specific of P. falciparum was detected, were also positive using paleo-autoimmunohistochemistry and metagenomics.Conclusion: Dental pulp erythrocytes proved to be suitable for the direct paleomicrobiology documentation of malaria in nine individuals buried in medieval Corsica, in agreement with historical data. This provides additional information on the millennial dynamics of Plasmodium spp. in the Mediterranean basin.
