CARDIAC GLYCOSIDES, PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY FROM PLANT CELL SUSPENSION CULTURES OF Thevetia peruviana
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1 décembre 2017
- handle: 10670/1.9vb5nn
info:eu-repo/semantics/openAccess
Mots-clés
Plant metabolites Anti-oxidant activity Reactive Oxygen Species Phenols Plant cell suspension culture Thevetia peruvianaSujets proches
Yellow oleander Apple, Tiger Cerbera thevetia Be-still tree Thevetia nereifolia Exile tree Thevetia neriifolia Oleander, Yellow Tiger apple Luckynut thevetia Phenolic compounds PhenolicsCiter ce document
Juan Pablo Arias et al., « CARDIAC GLYCOSIDES, PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY FROM PLANT CELL SUSPENSION CULTURES OF Thevetia peruviana », Revista U.D.C.A Actualidad & Divulgación Científica, ID : 10670/1.9vb5nn
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Résumé
Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) as well as sucrose consumption in 7 days. Preferential glucose over fructose consumption was observed. Intracellular production of cardiac glycosides reached 2.58mg DE/gDW at day 19. Highest extracellular production was reached between day 2 and 7 (6.19mg DE/L). Highest extracellular phenolic content was 80.61 ± 5.16mg GAE/L at day 7. Intracellular phenolic content increased to 2.76 ± 0.14mg GAE/gFW at day 7 and remained constant until day 19. ROS production at day 7 could be related to sucrose and glucose total consumption. Pearson Product-Moment Correlation Coefficient (ρ) showed that the phenolic compounds in cell suspension cultures of T. peruviana were responsible for the observed anti-oxidant activity. All together, these results give the first steps in metabolic behavior in cell suspension cultures of T. peruviana.