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2015
- handle: 10670/1.ar2ztd
- hal: hal-02630964
- doi: 10.1371/journal.pone.0140111
- PRODINRA: 332996
- WOS: 000363185500026
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info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0140111
http://creativecommons.org/licenses/by/ , info:eu-repo/semantics/OpenAccess
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Nutrition--Health aspects Alimentation Food--Health aspectsCiter ce document
Lenha Mobuchon et al., « Food deprivation affects the miRNome in the lactating goat mammary gland », HALSHS : archive ouverte en Sciences de l’Homme et de la Société, ID : 10.1371/journal.pone.0140111
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Résumé
Nutrition affects milk composition thus influencing its nutritional properties. Nutrition also modifies the expression of mammary genes, whose regulation is not fully understood. MicroRNAs (miRNA) are small non coding RNA which are important post-transcriptional regulators of gene expression by targeting messenger RNAs. Our goal was to characterize miRNA whose expression is regulated by nutrition in the lactating goat mammary gland, which may provide clues to deciphering regulations of the biosynthesis and secretion of milk components. Using high-throughput sequencing technology, miRNomes of the lactating mammary gland were established from lactating goats fed ad libitum or deprived of food for 48h affecting milk production and composition. High throughput miRNA sequencing revealed 30 miRNA with an expression potentially modulated by food deprivation; 16 were down-regulated and 14 were up-regulated. Diana-microT predictive tools suggested a potential role for several nutriregulated miRNA in lipid metabolism. Among the putative targets, 19 were previously identified as differently expressed genes (DEG). The functions of these 19 DEG revealed, notably, their involvement in tissue remodelling. In conclusion, this study offers the first evidence of nutriregulated miRNA in the ruminant mammary gland. Characterization of these 30 miRNA could contribute to a clearer understanding of gene regulation in the mammary gland in response to nutrition.