Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter
Fiche du document
1 septembre 2010
- handle: 10670/1.bh29uj
info:eu-repo/semantics/openAccess
Sujets proches
Flamingo flower Flamingo lilies Oilcloth flowers Anthurium andreanum Anthurium andraeanum Tailflowers Anthurium Lilies, Flamingo Tail flowersCiter ce document
Qing Zhao et al., « Optimization in Agrobacterium-mediated transformation of Anthurium andraeanum using GFP as a reporter », Electronic Journal of Biotechnology, ID : 10670/1.bh29uj
Métriques
Partage / Export
Résumé
Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to Agrobacterium-mediated transformation. We also optimized the concentration of AS added to co-cultivation media. Genomic PCR revealed that 11 of the 22 resistant plantlets regenerated on selective medium were successfully transformed. Green fluorescence was observed using a fluorescence microscope in 7 of the 11 PCR-positive plants, indicating GFP was expressed stably in the transformed Anthurium andraeanum. The highest transformation efficiency obtained in this study was 1.71% (percentage of explants with transgenic shoots in total explants) when callus explants were used as starting material and 125 μmol l-1 AS was added during the co-cultivation process.