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1 juillet 2007
- handle: 10670/1.bk18uu
Ce document est lié à :
10.4067/S0717-34582007000300015
info:eu-repo/semantics/openAccess
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groundnut isolation simple sequence repeatSujets proches
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Chuan Tang Wang et al., « Isolation of simple sequence repeats from groundnut », Electronic Journal of Biotechnology, ID : 10670/1.bk18uu
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SSRs have proved to be the most powerful tool for variety identification in groundnut of similar origin, and have much potential in genetic and breeding studies. To facilitate SSR discovery in groundnut, we proposed a highly simplified SSR isolation protocol based on multiple enzyme digestion/ligation, mixed biotin-labeled probes and streptavidin coated magnetic beads hybridization capture strategy. Of the 272 colonies randomly picked for sequencing, 119 were found to have unique SSR inserts