High-Throughput DNA sequencing of ancient wood

Stefanie Wagner; Frédéric Lagane; Andaine Seguin-Orlando; Mikkel Schubert; Thibault Leroy; Erwan Guichoux; Emilie Chancerel; Inger Bech-Hebelstrup; Vincent Bernard; Cyrille Billard; Yves Billaud; Matthias Bolliger; Christophe Croutsch; Frédérique Eynaud; Karl Uwe Heussner; Joachim Köninger; Fabien Langenegger; Frédéric Leroy; Christine Lima; Nicoletta Martinelli; Garry Momber; André Billamboz; Oliver Nelle; Antoni Palomo; Raquel Piqué; Marianne Ramstein; Roswitha Schweichel; Harald Stäuble; Willy Tegel; Xavier Terradas; Florence Verdin; Christophe Plomion; Antoine Kremer; Ludovic Orlando

High-Throughput DNA sequencing of ancient wood

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Discipline

Archéologie et Préhistoire

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Articles

Périmètre

Publications

Langue

Anglais

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ISIDORE Id: 10670/1.d124bd...
hal: hal-01783724
doi: 10.1111/mec.14514
PRODINRA: 423643
PUBMED: 29412519
WOS: 000429575900005

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HAL SHS (Sciences de l’Homme et de la Société)

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Ce document est lié à :
info:eu-repo/semantics/altIdentifier/doi/10.1111/mec.14514

Ce document est lié à :
info:eu-repo/semantics/altIdentifier/pmid/29412519

Ce document est lié à :
info:eu-repo/grantAgreement/EC/FP7/339728/EU/From Holocene to Anthropocene: the pace of microevolution in trees/TREEPEACE

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Archives ouvertes

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Centre pour la communication scientifique directe

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info:eu-repo/semantics/OpenAccess

Mots-clés En Und

oak Quercus petraea/robur chloroplast DNA ancient DNA chloroplast degradation temperate trees

Sujets proches En

Deoxyribonucleic acid TNA (Nucleic acid) Desoxyribonucleic acid Thymonucleic acid

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Stefanie Wagner et al., « High-Throughput DNA sequencing of ancient wood », HAL SHS (Sciences de l’Homme et de la Société), ID : 10.1111/mec.14514

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Reconstructing the colonization and demographic dynamics that gave rise to extant forests is essential to forecasts of forest responses to environmental changes. Classical approaches to map how population of trees changed through space and time largely rely on pollen distribution patterns, with only a limited number of studies exploiting DNA molecules preserved in wooden tree archaeological and subfossil remains. Here, we advance such analyses by applying high-throughput (HTS) DNA sequencing to wood archaeological and subfossil material for the first time, using a comprehensive sample of 167 European white oak waterlogged remains spanning a large temporal (from 550 to 9,800 years) and geographical range across Europe. The successful characterization of the endogenous DNA and exogenous microbial DNA of 140 (~83%) samples helped the identification of environmental conditions favouring long-term DNA preservation in wood remains, and started to unveil the first trends in the DNA decay process in wood material. Additionally, the maternally inherited chloroplast haplotypes of 21 samples from three periods of forest human-induced use (Neolithic, Bronze Age and Middle Ages) were found to be consistent with those of modern populations growing in the same geographic areas. Our work paves the way for further studies aiming at using ancient DNA preserved in wood to reconstruct the micro-evolutionary response of trees to climate change and human forest management.

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High-Throughput DNA sequencing of ancient wood

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