Prospective evaluation of RT-PCR on sputum versus culture, urinary antigens and serology for Legionnaire's disease diagnosis
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- handle: 10670/1.hwyz9u
- hal: hal-02100285
- doi: 10.1016/j.jinf.2016.04.039
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info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jinf.2016.04.039
Mots-clés
Legionella pneumophila Legionnaires' disease Legionellosis Diagnosis PCR Pneumonia Bacteriological methodsSujets proches
Medical examinations (Diagnosis) Diseases--Diagnosis Diseases--Testing Examinations, Medical (Diagnosis) Medical diagnosis Medical tests (Diagnosis)Citer ce document
Elisabeth Botelho-Nevers et al., « Prospective evaluation of RT-PCR on sputum versus culture, urinary antigens and serology for Legionnaire's disease diagnosis », HAL-SHS : géographie, ID : 10.1016/j.jinf.2016.04.039
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ObjectivesLegionnaires' disease (LD) is a severe disease associated with community and hospital-acquired pneumonia, frequently under diagnosed. The main aim of our study was to determine the value of PCR for the diagnosis of LD in routine clinical practice.MethodsIn a prospective study, from March 2007 to April 2010, the value of PCR on non-invasive respiratory specimens (NIRS) was compared to those of the other available tools for LD diagnosis in patients hospitalized for pneumonia.ResultsAmong 254 consecutive cases of pneumonia included, 24 cases were LD (19 confirmed and 5 probable) representing the first documented microbiological etiology. Molecular diagnosis of LD was performed on NIRS by using 16S rRNA PCR, and secondarily mip PCR, with no discrepant results between the 2 methods: it was found positive in 14 cases and led to identify 2 supplementary probable cases of LD. Based on clinical and at least 2 positive LD tests, PCR yielded a better diagnostic value than antigen urinary test (12 vs 10 cases).ConclusionThese results revealed that molecular diagnosis of LD on NIRS is reliable and may contribute to better identify cases of LD.